Extranodal oral non-Hodgkin's lymphomas. A retrospective study of 40 cases in Argentina.

A retrospective study was conducted of extranodal oral Non-Hodgkin's Lymphomas diagnosed at the Surgical Pathology Laboratory of the School of Dentistry at Buenos Aires University, Argentina, between 1985 and 2004. The 40 cases found represent 0.2% of the oral biopsies diagnosed during that time and 4.6% of malignant neoplasias. Overall mean age of patients was 49.4 years, and frequency was greater in males. 80% affected soft tissues. Prevalent location was gingival, followed by palate. Intraosseous cases were more frequent in mandible (75%) than in upper maxilla. 100% of the cases were phenotype B, with a higher frequency of high-grade aggressiveness. The most common histological type was Diffuse Large Cell Lymphoma. 60% of the Plasmablastic Lymphomas in the series came from HIV+ patients. Evolution time prior to consultation was 1 to 3 months in 57.7% of the cases.

Agnor as a marker of malignant transformation in odontogenic keratocysts.

Nucleolar Organizer Region (AgNOR) counts have been reported as a useful, complementary diagnostic tool for several neoplasias. The morphometric evaluation of AgNOR has also been shown to contribute to the detection of incipient cellular alterations. The aim of the present study was to perform a morphometric analysis of AgNOR in keratocysts (KC), and in three rare cases of malignant transformation of keratocysts (KCm) to explore their use as a marker of the process of malignant transformation in this entity. We performed a study of the 3 cases of keratocysts that had suffered transformation into squamous cell carcinoma (SCC) that were available in our files and of 8 cases of solitary keratocysts (KCs). Paraffin sections were silver stained and employed to perform a morphometric analysis of 7 parameters related to number, size and shape of AgNORs. A comparative analysis of the epithelium of solitary keractocyts (KCs), the cystic epithelium adjacent to the area of transformation (KCm) and the epithelium of the SCC cords was performed. The epithelia of KCs and KCm exhibited statistically significant differences for four of the parameters analyzed: nuclear volume (VNUC), single AgNOR volume (VNOR), Total AgNOR per nucleus (TVNOR) and Contour Index of nuclei (CINUC). KCs and SCC exhibited statistically significant differences for all of the parameters. Given the ease with which this technique can be applied to routine histopathological material, we propose AgNOR evaluation as a diagnostic and prognostic aid in cystic entities that are suspected to have a neoplastic potential.

Agnor as a marker of malignant transformation in odontogenic keratocysts.

Nucleolar Organizer Region (AgNOR) counts have been reported as a useful, complementary diagnostic tool for several neoplasias. The morphometric evaluation of AgNOR has also been shown to contribute to the detection of incipient cellular alterations. The aim of the present study was to perform a morphometric analysis of AgNOR in keratocysts (KC), and in three rare cases of malignant transformation of keratocysts (KCm) to explore their use as a marker of the process of malignant transformation in this entity. We performed a study of the 3 cases of keratocysts that had suffered transformation into squamous cell carcinoma (SCC) that were available in our files and of 8 cases of solitary keratocysts (KCs). Paraffin sections were silver stained and employed to perform a morphometric analysis of 7 parameters related to number, size and shape of AgNORs. A comparative analysis of the epithelium of solitary keractocyts (KCs), the cystic epithelium adjacent to the area of transformation (KCm) and the epithelium of the SCC cords was performed. The epithelia of KCs and KCm exhibited statistically significant differences for four of the parameters analyzed: nuclear volume (VNUC), single AgNOR volume (VNOR), Total AgNOR per nucleus (TVNOR) and Contour Index of nuclei (CINUC). KCs and SCC exhibited statistically significant differences for all of the parameters. Given the ease with which this technique can be applied to routine histopathological material, we propose AgNOR evaluation as a diagnostic and prognostic aid in cystic entities that are suspected to have a neoplastic potential.